IgE against plant / insect CCDs is blocked by an artificial, polyvalent inhibitor that specifically reduces these unwanted reactivities while leaving untouched all other IgE interactions. Production of this blocker involves proteolytic degradation of the biogenic starting material and extensive purification of the hapten structure. Hence, unlike natural glycoproteins this CCD-blocker offers highest certainty of absence of peptide epitopes that could cause unintended inhibitory events. Coupling of the haptens to human serum albumin finally provides multivalency required for efficient competitive inhibition. Owing to the blockers high efficacy, virtually no dilution of the serum occurs. The proglycan CCD-blocker is considered applicable to every assay system for allergen-specific IgE and has so far been successfully applied with several multi-allergen strips or array formats as well as with the Phadia CAP system.
The blocker is applied to serum in a volume ratio of 1 to 50. Thus, essentially no dilution of the serum occurs. After a few minutes of equilibration time, the usual procedure for detection and quantification of allergen specific IgE can be conducted.
The figure below exemplifies the effect of the CCD-blocker on a multiallergen test strip. (for details see Holzweber et al. 2013, Inhibition of IgE binding to cross-reactive carbohydrate determinants enhances diagnostic selectivity. Allergy 68, 1269-77).
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